Study of cervical precancerous lesions detection by spectroscopy and support vector machine.

BACKGROUND AND OBJECTIVE: Diffuse reflectance spectroscopy (DRS) offers a fast, non-invasive, and low-cost alternative for cervical cancer diagnosis. We aim to develop a method for screening precancerous lesions based on DRS. MATERIAL AND METHODS: Characteristic parameters of cervical tissue were extracted from spectra, including optical characteristic parameters such as absorption and scattering coefficients, and some slope and area parameters of the spectrum. Data were randomly divided into training (60%) and test (40%) sets. Of the 210 included patients, 166 were healthy, 22 had erosion of the cervix, and 31 had cervical intraepithelial neoplasia (CIN). The support vector machine (SVM) algorithm was used to classify normal and abnormal cervical tissue based on 11 characteristic parameters. RESULTS: The SVM with linear kernel function, applied on the training data, could distinguish tissue with lesions from healthy tissue with an accuracy of 1.00. When the classifiers were applied to the test set, erosion of cervix and CIN could be discriminated from healthy tissue with an accuracy of 0.95 (±0.03). CONCLUSIONS: This research shows that the diagnostic algorithm can be valuable for non-invasive diagnosis of cervical cancer. This is a significant step toward the development of a tool for tissue assessment of cervical cancer.

[Development of Diffuse Reflectance Spectroscopy Detection and Analysis System for Cervical Cancer].

Cervical cancer is a common female malignant tumor. It has been increasing and rejuvenating in recent years. Early screening of cervical cancer is an effective control method to block cancer. In this study, a diffuse reflectance spectrum detection and analysis system based on LabWindows development software and MariaDB database was developed, which can acquire and save the spectral data to the database. The method of a neural network model based on spectral database was built to distinguish the cervical tissue and the normal tissue. The nude mouse tumor model test and human volunteer test were performed respectively, which verified that the system can distinguish between normal tissue and tumor tissue, and can be applied to the screening of cervical precancerous lesions.

[Development of Blood Flow and Oxygen Monitoring System for Mice Based on Laser Speckle and Spectrum].

In vivo simultaneous monitoring of blood flow and changes of concentration of oxyhemoglobin (ΔHbO2) in brain is a key important method for the research of cerebrovascular disease. In this study, a new monitoring system, combining laser speckle contrast imaging method and spectral analysis method, was proposed, which could be utilized to measure the cerebral blood flow and ΔHbO2 on mice during traumatic brain injury. The principle of the present system was studied and the hardware platform of the detection system was built. Then, user interface software and algorithms were implemented based on Labview and Matlab software. Finally, the performance of the present system was verified by the in vivo experiments.

Effects of puerarin on spatial learning and memory function in mice with acute alcohol consumption: An evaluation based upon firing rate and oxygen saturation analysis.

BACKGROUND: Ethanol is associated with various medical comorbidities affecting the brain and central nervous system. OBJECTIVES: The use of puerarin in treating alcohol-induced memory disorders is systematically evaluated in this study based upon an analysis of the firing rate and oxygen saturation (SO2). MATERIAL AND METHODS: A multi-channel data acquisition system and near-infrared spectroscopy (NIRS) were combined to obtain the electroneurophysiological signals and SO2 in the hippocampus of mice from the Institute of Cancer Research (ICR) after ethanol injection. A T-maze test was performed to study the alteration of spatial memory function. Ethanol was administered intraperitoneally (i.p.) in 2 dosages (1.5 g/kg and 0.5 g/kg). To investigate the effects of puerarin against acute ethanol-induced memory impairment, the same parameters corresponding to electroneurophysiological signals, SO2 and behavior performance in mice were also recorded upon the supplementation of puerarin in 2 dosages (25 mg/kg and 50 mg/kg). RESULTS: An inhibited firing rate and decreased SO2 were found in mice treated with an ethanol dose of 1.5 g/kg, which resulted in a low correct choice rate in the T-maze test, while an opposite trend appeared in mice which had been administered a moderate ethanol dose (0.5 g/kg). The trend of SO2 was positively correlated with that of the firing rate. A decreased firing rate and SO2 were accompanied by a decrease in the correct choice rate. With the supplementation of puerarin, a significant increase in the mean firing rate and SO2, as well as an improved correct choice rate, can be found in mice injected with excessive ethanol. CONCLUSIONS: The electroneurophysiological signals and NIRS were combined for the first time to prove that an excessive intake of ethanol can inhibit the spatial learning and memory function of mice. The supplementation of puerarin can suppress these adverse effects induced by ethanol at a high dosage, as evidenced by the increased firing rate and SO2.

[Development of the Intracranial Pressure and Intracranial Temperature Monitor and Experimental Research on Trauma].

OBJECTIVES: A set of intracranial pressure and intracranial temperature monitor was developed. Moreover, it was verified to be effective in the monitoring of intracranial parameters by designed experiments. METHODS: The intracranial pressure and intracranial temperature monitor was tested in the water bath comparing with the Codman intracranial pressure monitor and mercury thermometers. As well, the monitor was applied in the monitoring of rat brain edema in vivo. RESULTS: The maximum error is less than 266.64 Pa in the intracranial pressure measurement compared to the Codman intracranial pressure monitor, and the maximum error is less than 0.3 oC in the temperature measurement according to mercury thermometers. Furthermore, the monitor could real-time obtain the intracranial pressure and intracranial temperature in the brain edema in vivo. CONCLUSIONS: The intracranial pressure and intracranial temperature monitor realizes the real-time in vivo monitoring of intracranial pressure and intracranial temperature. The measurement accuracy meets the acquirement of doctors. The instrument has potential for clinical use.